Sains Malaysiana 55(10)(2026): 1664-1680

http://doi.org/10.17576/jsm-2026-5510-06

 

Untargeted Muscle Tissue Metabolites Profiling in an Ageing Animal Model Supplemented with Ginger Active Compounds

(Profil Metabolit Tisu Otot Tidak Bersasar dalam Model Haiwan Penuaan yang Disuplementasi dengan Sebatian Aktif Halia)

 

NUR FATIN NABILAH MOHD SAHARDI¹, NUR FATHIAH ABDUL SANI², JEN KIT TAN^2,4, MARIAM FIRDHAUS MAD NORDIN³ & SUZANA MAKPOL^2,4,*

 

¹Secretariat Research and Innovation, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia

²Department of Biochemistry, Faculty of Medicine, Level 17 Preclinical Building, Universiti KebangsaanMalaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia

³AM Zaideen Ventures Sdn Bhd, Kuala Lumpur, Malaysia

⁴Ageing and Degenerative Diseases Research Group, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

Diserahkan: 22 April 2026/Diterima: 23 September 2026

 

*Pengarang untuk surat-menyurat; email: suzanamakpol@ukm.edu.my

 

Abstract

Age-related muscle loss is a significant health concern among older adults, contributing to frailty, reduced strength, and functional decline, with oxidative stress identified as a key underlying driver of this deterioration. Ginger (Zingiber officinale Roscoe), a traditional herb with established antioxidant properties, has shown potential to mitigate oxidative damage. However, its effects on muscle metabolic regulation during the ageing process remain largely unexplored. Therefore, this study aimed to investigate the effects of ginger extract on metabolic pathways associated with age-related muscle loss. Sprague-Dawley rats of various ages (young, adult, and old) were administered distilled water or ginger extract (200 mg/kg body weight) daily for three months. Muscle tissues were subjected to untargeted metabolomic profiling using UHPLC-MS/MS. Correlation analyses were conducted to determine associations between significantly altered metabolites and oxidative stress and inflammatory markers. Untargeted metabolomic profiling showed that ageing markedly altered muscle metabolites, particularly those in the phenylalanine, tyrosine, and tryptophan biosynthesis pathway. Ginger supplementation partially reversed these age-associated shifts; most notably, it significantly downregulated β-muricholic acid and xanthosine in old rats, which had increased during ageing. Furthermore, ginger treatment upregulated γ-L-glutamyl-L-glutamic acid, suggesting enhanced antioxidant capacity and muscle regenerative pathways. These findings suggest that ginger extract may attenuate age-related physiological decline by modulating several key metabolites disrupted during ageing, highlighting its potential as a supplement to support healthy ageing in older individuals.

Keywords: Ginger extract; metabolomic study; muscle ageing; oxidative stress

 

Abstrak

Kehilangan otot berkaitan usia merupakan isu kesihatan yang signifikan dalam kalangan warga emas yang menyumbang kepada kerapuhan, penurunan kekuatan dan kemerosotan fungsi, dengan tekanan oksidatif dikenal pasti sebagai pemacu utama kepada kemerosotan otot. Halia (Zingiber officinale Roscoe), sejenis herba tradisional yang mempunyai sifat antioksidan yang terbukti, telah menunjukkan potensi dalam mengurangkan kerosakan oksidatif. Walau bagaimanapun, kesannya terhadap regulasi metabolik otot semasa proses penuaan masih belum banyak dikaji. Oleh itu, penyelidikan ini bertujuan untuk mengkaji kesan ekstrak halia terhadap laluan metabolik yang berkaitan dengan kehilangan otot akibat penuaan. Tikus Sprague-Dawley daripada pelbagai peringkat umur (muda, dewasa dan tua) diberikan air suling atau ekstrak halia (200 mg/kg berat badan) setiap hari selama tiga bulan. Tisu otot dianalisis menggunakan pemprofilan metabolik tidak bersasar dengan UHPLC-MS/MS. Analisis korelasi dijalankan untuk menentukan hubungan antara metabolit yang berubah secara signifikan dengan penanda tekanan oksidatif dan keradangan. Hasil pemprofilan metabolomik tidak bersasar menunjukkan bahawa penuaan secara ketara mengubah metabolit otot, khususnya dalam laluan biosintesis fenilalanina, tirosin dan triptofan. Suplementasi halia didapati sebahagiannya membalikkan perubahan berkaitan usia ini; yang paling ketara, ia menurunkan secara signifikan asid β-murikolik dan xantosin dalam tikus tua, yang meningkat semasa proses penuaan. Selain itu, rawatan halia meningkatkan γ-L-glutamil-L-asid-glutamik, yang mencadangkan peningkatan kapasiti antioksidan dan laluan regenerasi otot. Keputusan ini menunjukkan bahawa ekstrak halia berpotensi memperlahankan kemerosotan fisiologi berkaitan usia dengan memodulasi beberapa metabolit utama yang terganggu semasa proses penuaan, sekali gus menonjolkan potensinya sebagai suplemen untuk menyokong penuaan sihat dalam kalangan individu berusia.

Kata kunci: Ekstrak halia; kajian metabolik; penuaan otot; tekanan oksidatif

 

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